Objective To construct an online nursing consultation service model and to verify its practice effects. Methods Based on the hospital′s quality resources, a nursing service team was formed to provide online nursing consultation service, then the online service contents and process were formulated in combination with actual needs of the people. The number of online consultations, satisfaction of the customers, and nurses′ perceived professional benefits were measured to evaluate the practice effects. Results From 21st January to 2nd August 2020, a total of 3 847 patients received online nursing services, with the satisfaction rate of 97.79%. There were significant differences in total score and dimension scores of perceived professional benefits among nursing staff before and after implementing the service (P<0.01 for all). Conclusion Implementing online nursing consultation service can meet needs of different people and enhance their satisfaction, as well as promote nurses′ perceived professional benefits.