Objective To observe the intervention effect of auricular point therapy combining with "He" character sound on perioperative stress response for patients with uterine fibroids and undergoing laparoscopic total hysterectomy （TH）. Methods A total of 90 patients undergoing laparoscopic TH were randomized into a control group, auricular point group and combined group, with 30 cases in each group.The control group received routine perioperative nursing, the auricular point group additionally received auricular point therapy at one day before the operation, and the combined group received "He" character sound breathing training on the basis of the auricular point group, which was lasted from one day before the operation to 3 days after the operation. The heart rate and blood pressure of the three groups at different time points were recorded respectively, and the patients′ anxiety level was evaluated before the intervention, at the night before the operation and after the intervention. Results There were significant differences in time effect and group effect of systolic pressure,diastolic pressure and heart rate at different time points among the three groups （P＜0.01 for all）.When compared with the control group and the auricular point group, the anxiety scores of the combined group at the night before the operation and after the intervention were statistically lower （P＜0.05,P＜0.01）. Conclusion Auricular point therapy combining with "He" character sound can effectively relieve perioperative anxiety, maintain blood pressure and heart rate at a stable level, and reduce stress response for patients undergoing total hysterectomy.