Objective To explore prevention and control measures for medical staff working in a hospital designated for treatment of coronavirus disease 2019(COVID-19) and examine the effcet of the measures. Methods A three-level hospital infection prevention and control committee was established in the designated hospital. Diversified training was carried out, strict prevention and control measures and supervision were proceeded throughout the whole process, and various policies and logistics support were delivered. Results A total of 2 454 patients with COVID-19 pneumonia were treated and 525 were cured and discharged. No medical staffer was infected with the contagion. Conclusion The establishment and implementation of the three-level prevention and control mechanism for nosocomial infections is a safety guarantee for medical personnel to avoid nosocomial infections. It also enhances treatment efficacy. 