Objective To describe the level of adversity quotient of male nurses in tertiary hospitals in Luzhou, to identify its influencing factors, and to provide reference for targeted intervention. Methods Totally, 228 male nurses from 5 tertiary hospitals in Luzhou were surveyed using the Chinese version of Adversity Quotient Scale. Results The total score of adversity quotient of male nurses was 133.75±19.36. Multiple regression analysis indicated position, marital status and whether he was the only child in the family were the influencing factors of adversity quotient (P＜0.05,P＜0.01). Conclusion Male nurses report a moderate level of adversity quotient, and those who are unmarried, who have no siblings, and who don′t undertake management task, report poor adversity quotient. Hospital mangers should strengthen targeted training and provide opportunity for career development to enhance adversity quotient of male nurses.