Objective To test the applicability of the simplified Chinese version of the Aging Males′ Symptoms Scale (AMS) in prostate cancer patients receiving hormonal therapy. Methods The traditional Chinese version of AMS was culturally adapted to form the simplified Chinese version, then 10 experts were invited to evaluate the content validity. After that, 221 prostate cancer patients receiving hormonal therapy were investigated to test the reliability and validity of the simplified Chinese version of AMS. Results The content validity index for the scale was 0.95. The scale contained 17 items, and 3 factors were identified by exploratory factor analysis, accounting for 60.801% of the total variance. The Cronbach′s alpha coefficient of the total scale was 0.871 and the split-half reliability was 0.748. Conclusion The simplified Chinese version of AMS has good reliability and validity, and can be used as a symptom assessment tool for androgen deficiency related adverse reactions in prostate cancer patients receiving hormonal therapy.