Objective To retrieve and analyze the best evidence for the practice of artificial airway suctioning in patients with brain injury, and to provide evidence for artificial airway nursing care. Methods Aiming at forming a best practice guide for artificial airway suctioning in patients with brain injury, we retrieved domestic and foreign literature by computerized search. Then we evaluated the quality of studies and determined the level of evidence by referencing to JBI evidence appraisal and recommendation system. Results A total of 18 pieces of evidence regarding artificial airway suctioning for patients with brain injury were summarized, including such six aspects as suction indications, patient assessment and preparation, suction methods, procedures, and infection control. Conclusion The best evidence summary for artificial airway suctioning in patients with brain injury could standardize the practice of artificial airway suctioning in clinical settings. During the application of the evidence, clinical staff should consider the actual situation of patients to make comprehensive assessment, and reduce suction-related damage to the injured brain as much as possible.