Objective To establish a relatively complete micro-lecture group network platform for clinical specialized nursing skills, and to explore the effect of applying the platform in the courses of obstetrics and gynecological nursing. Methods We established micro-lecture group network platform for specialized nursing skills in such clinical fields as medical nursing, surgical nursing, obstetrics and gynecological nursing, and emergency and critical care. The nursing undergraduates enrolled in 2016 to a medical college in Shandong province were divided into an intervention group and a control group.The control group (n=208) was subjected to traditional teaching model, while the intervention group (n=212) to teaching based on the microlecture group network platform. The theory and skill examinations scores of the two groups were compared, and the attitudes of the intervention group students towards the application of micro-lecture group network platform were surveyed. Results The theory and skill examinations scores of the intervention group were higher than those of the control group (P<0.01 for both). During the survey, 89.15% of the intervention group students preferred micro-lecture, and 86.79% hoped to use microlecture in other clinical courses. Conclusion The application of micro-lecture group network platform for clinical specialized nursing skills is helpful to improve the teaching effect.