Objective To improve nurses′ adherence to practice of checking during the administration of medicines, and to ensure medication safety. Methods A comprehensive intervention program was designed and implemented, including 5 steps of introduction of patient safety and checking, and then perception,learning, externalization, and clinical practice. A total of 623 nurses were randomly selected from 32 departments to measure the effect after 6 months of intervention. Results The overall adherence to practice of checking during administration of medicines and the adherence to practice of check during managing medical order, placing medicines, preparing medicines and administrating medicines, as well as the use of personal digital assistant (PDA) in checking were significantly enhanced after the intervention (P＜0.01 for all). Conclusion Implementing intervention measures designed from nurses′ perspectives can effectively enhance PDA use in checking and adherence to practice of checking during the whole administration process, thus to ensure medication safety.