Objective To systematically retrieve and assess the quality of clinical practice guidelines (CPGs) regarding management of venipuncture pain in children, and to provide suggestions for clinical practice. Methods All CPGs pertaining to venipuncture in children issued between January 2009 and October 2018 were searched in electronic databases and related websites of guidelines, then the included CPGs were evaluated using the Chinese version of the Appraisal of Guidelines for Research and Evaluation (AGREE) II. Results We identified 5 eligible CPGs.The domain of scope and purpose in the AGREE II scored the highest （92.24±3.44）%, followed by stakeholder involvement （86.62±6.63）% and clarity of presentation （82.94±7.23）%. Twenty-three recommendations were summarized into the following categories: pain assessment, measurement, management and intervention. Conclusion The CPGs pertaining to management of venipuncture pain in pediatric patients is limited, and the domains of rigor of development and applicability need to be improved. There is no Chinese CPG regarding this topic, so we can introduce well-accepted CPGs and conduct cross-cultural adaptation, thus to guide evidence-based practice for management of venipuncture pain in pediatric patients.