Objective To explore the effect of standardized warning applied in management of interruptions in clinical nursing practice. Methods We analyzed the causes, types and adverse outcomes of interruptions in nursing practice from the preliminary study to develop items of standardized warning, then explained to nurses and conducted inspection and supervision. The incidence of interruptions and adverse events induced by interruptions were compared before and after the intervention. Results The incidence of interruptions and adverse events induced by interruptions were significantly reduced after the intervention (P<0.01 for both). Conclusion Design and implementation of the standardized warning can reduce interruptions in clinical nursing practice.